PrimePCR™ Probe Assay: CKLF-CMTM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best coverage

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CKLF-CMTM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CKLF-CMTM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058564
Assay Design:   exonic
Chromosome Location:   16:66612917-66613023question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000527729 ENST00000529718 ENST00000511072 ENST00000378391 ENST00000514189 ENST00000270722 ENST00000512462 ENST00000509860 ENST00000378398 ENST00000441472 ENST00000442529 ENST00000332695 ENST00000336328 ENST00000528324 ENST00000531885 ENST00000457188 ENST00000533666 ENST00000533953 ENST00000379500 ENST00000328020 ENST00000317775 ENST00000338101 ENST00000344089 ENST00000252136 ENST00000403707 ENST00000404751 ENST00000439169 ENST00000519973

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring CKLF (chemokine-like factor) and CMTM1 (CKLF-like MARVEL transmembrane domain containing 1) genes on chromosome 16. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq Feb 2011]

Gene Symbol:   CKLF-CMTM1
Gene Name:   CKLF-CMTM1 readthrough
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000254788
Entrez:   100529251
Chromosome Mapping:   16q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 35.620000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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