PrimePCR™ Probe Assay: SCAND1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057939
Assay Design:   exonic
Chromosome Location:   20:34541996-34542194question
Amplicon Length:   169
Splice Variants Targeted:   ENST00000569785 ENST00000413360 ENST00000273582 ENST00000305978 ENST00000373991 ENST00000264832 ENST00000328333 ENST00000454817 ENST00000306749 ENST00000594202 ENST00000596536 ENST00000539407 ENST00000414982 ENST00000600737 ENST00000576346 ENST00000407967 ENST00000319176 ENST00000392549 ENST00000599174 ENST00000316793 ENST00000450053 ENST00000383740 ENST00000586421 ENST00000566228 ENST00000306030 ENST00000508939 ENST00000397501 ENST00000346049 ENST00000517339 ENST00000338238 ENST00000544172 ENST

Gene Information

Description Not Available

Gene Symbol:   SCAND1
Gene Name:   SCAN domain containing 1
Aliases:   RAZ1, SDP1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000171222
Entrez:   51282
UniGene:   Hs.584909
Chromosome Mapping:   20q11.1-q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 41.930000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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