PrimePCR™ Probe Assay: VCX3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VCX3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VCX3B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057869
Assay Design:   exonic
Chromosome Location:   X:8432879-8432989question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000381089 ENST00000398729 ENST00000317103 ENST00000381032 ENST00000453306 ENST00000444481 ENST00000393750

Gene Information

This gene belongs to the VCX/Y gene family which has multiple members on both X and Y chromosomes and all are expressed exclusively in male germ cells. The X-linked members are clustered on chromosome Xp22 and the Y-linked members are two identical copies of the gene within a palindromic region on chromosome Yq11. The family members share a high degree of sequence identity with the exception that a 30-nt unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. The VCX gene cluster is polymorphic in terms of copy number; different individuals may have a different number of VCX genes. This family member as represented by the reference genome allele contains 14 copies of the 30-nt repeat unit. VCX/Y genes encode small and highly charged proteins containing putative bipartite nuclear localization signals. Although the exact function of this family member has yet to be determined a role in mRNA stability regulation can be inferred from the ability of the highly similar family member VCX-A to inhibit mRNA decapping. A possible role in the regulation of ribosome assembly during spermatogenesis has also been suggested. [provided by RefSeq Aug 2010]

Gene Symbol:   VCX3B
Gene Name:   variable charge, X-linked 3B
Aliases:   MGC119815, VCX-C, VCXC
RefSeq:   NC_000023.10 NT_167197.1
Ensembl:   ENSG00000205642
Entrez:   425054
Chromosome Mapping:   Xp22.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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