PrimePCR™ Probe Assay: EXOSC6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EXOSC6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EXOSC6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055944
Assay Design:   exonic
Chromosome Location:   16:70284197-70284303question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000435634

Gene Information

This gene product constitutes one of the subunits of the multisubunit particle called exosome which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known however it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs) but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs thereby promoting their rapid degradation. [provided by RefSeq Jul 2008]

Gene Symbol:   EXOSC6
Gene Name:   exosome component 6
Aliases:   EAP4, MTR3, Mtr3p, hMtr3p, p11
RefSeq:   NC_000016.9 NG_023191.1 NT_010498.15
Ensembl:   ENSG00000223496
Entrez:   118460
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.380000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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