PrimePCR™ Probe Assay: GNRHR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055820
Assay Design:   exonic
Chromosome Location:   1:145510028-145510170question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000361928 ENST00000312753 ENST00000581100 ENST00000581539

Gene Information

Not Available

Gene Symbol:   GNRHR2
Gene Name:   gonadotropin releasing hormone receptor 2, pseudogene [Source:HGNC Symbol;Acc:HGNC:16341]
Aliases:   GnRH-II-R
RefSeq:   Not Available
Ensembl:   ENSG00000211451
Entrez:   114814
UniGene:   Hs.711567
Chromosome Mapping:   1q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.490000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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