PrimePCR™ Probe Assay: MT1H, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055767
Assay Design:   exonic
Chromosome Location:   16:56703751-56704442question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000544264 ENST00000164024 ENST00000252971 ENST00000530544 ENST00000402868 ENST00000330479 ENST00000402034 ENST00000546530 ENST00000261729 ENST00000446861 ENST00000548055 ENST00000358743 ENST00000347557 ENST00000404574 ENST00000296473 ENST00000417270 ENST00000530438 ENST00000327111 ENST00000239462 ENST00000542466 ENST00000370060 ENST00000370055 ENST00000361699 ENST00000543994 ENST00000370057 ENST00000538883 ENST00000361981 ENST00000333206 ENST00000420878 ENST00000310677 ENST00000554068 ENST

Gene Information

Description Not Available

Gene Symbol:   MT1H
Gene Name:   metallothionein 1H
Aliases:   MGC70702, MT1
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000205358
Entrez:   4496
UniGene:   Hs.438462
Chromosome Mapping:   16q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.