PrimePCR™ Probe Assay: PABPN1L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PABPN1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PABPN1L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055744
Assay Design:   exonic
Chromosome Location:   16:88930785-88931432question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000594151 ENST00000600603 ENST00000601638 ENST00000599732 ENST00000601675 ENST00000221557 ENST00000391842 ENST00000309880 ENST00000389617 ENST00000498315 ENST00000381848 ENST00000361417 ENST00000356063 ENST00000420323 ENST00000218343 ENST00000397189 ENST00000327797 ENST00000479054 ENST00000345716 ENST00000527990 ENST00000254579 ENST00000423565 ENST00000354475 ENST00000344400 ENST00000223127 ENST00000562374 ENST00000448039 ENST00000297954 ENST00000432730 ENST00000411624 ENST00000395477 ENST

Gene Information

Description Not Available

Gene Symbol:   PABPN1L
Gene Name:   poly(A) binding protein, nuclear 1-like (cytoplasmic)
Aliases:   PABPNL1, ePABP2
RefSeq:   NG_008667.1 NC_000016.9 NT_010542.15
Ensembl:   ENSG00000205022
Entrez:   390748
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.630000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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