PrimePCR™ Probe Assay: HSPB9, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055485
Assay Design:   exonic
Chromosome Location:   17:40275005-40275094question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000372172 ENST00000372168 ENST00000585732 ENST00000585708 ENST00000586184 ENST00000588077 ENST00000587995 ENST00000382722 ENST00000331243 ENST00000295092 ENST00000381848 ENST00000355067 ENST00000268876 ENST00000394570 ENST00000591048 ENST00000433649 ENST00000378449 ENST00000602795 ENST00000344018 ENST00000299441 ENST00000238936 ENST00000396801 ENST00000355772 ENST00000396799 ENST00000417772 ENST00000376170 ENST00000376142 ENST00000359188 ENST00000376139 ENST00000376160 ENST00000376166 ENST

Gene Information

Description Not Available

Gene Symbol:   HSPB9
Gene Name:   heat shock protein, alpha-crystallin-related, B9
Aliases:   CT51, FLJ27437
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000197723
Entrez:   94086
UniGene:   Hs.620611
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.