PrimePCR™ Probe Assay: ZNF765, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055341
Assay Design:   exonic
Chromosome Location:   19:53905437-53911045question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000359798 ENST00000465448 ENST00000486474 ENST00000461321 ENST00000391785 ENST00000301093 ENST00000593941 ENST00000540331 ENST00000602162 ENST00000319783 ENST00000595798 ENST00000601643 ENST00000600943 ENST00000543227 ENST00000540744 ENST00000453741 ENST00000338230 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST00000390651 ENST00000434371 ENST00000549216 ENST00000391777 ENST00000601857 ENST00000595091 ENST00000458035 ENST00000474037 ENST00000467003 ENST00000396408 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF765
Gene Name:   zinc finger protein 765
Aliases:   DKFZp686J2252
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000196417
Entrez:   91661
UniGene:   Hs.685640
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.010000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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