PrimePCR™ Probe Assay: CD300E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055092
Assay Design:   exonic
Chromosome Location:   17:72613418-72613555question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000392619 ENST00000412268 ENST00000426295 ENST00000328630

Gene Information

CD300LE is an activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells. It mediates activating signals by interacting with DAP12 (TYROBP; MIM 604142) (Aguilar et al. 2004 [PubMed 15557162]).[supplied by OMIM Mar 2008]

Gene Symbol:   CD300E
Gene Name:   CD300e molecule
Aliases:   CD300LE, CLM-2, CLM2, CMRF35-A5, IREM-2, IREM2, PIgR-2, PIgR2
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000186407
Entrez:   342510
UniGene:   Hs.158954
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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