PrimePCR™ Probe Assay: PARP10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PARP10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PARP10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054583
Assay Design:   exonic
Chromosome Location:   8:145060142-145060311question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000524918 ENST00000313028 ENST00000525773 ENST00000313059 ENST00000525879 ENST00000528625 ENST00000525486 ENST00000531537 ENST00000529842 ENST00000528914 ENST00000528136 ENST00000529311 ENST00000530478 ENST00000532311 ENST00000531707 ENST00000565818 ENST00000568154 ENST00000562335 ENST00000563476 ENST00000567233 ENST00000567115 ENST00000565461 ENST00000569866 ENST00000564077 ENST00000565651 ENST00000563274 ENST00000567290 ENST00000562950 ENST00000564597 ENST00000570048 ENST00000557413 ENST

Gene Information

Poly(ADP-ribose) polymerases (PARPs) such as PARP10 regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al. 2005 [PubMed 15674325]).[supplied by OMIM Mar 2008]

Gene Symbol:   PARP10
Gene Name:   poly (ADP-ribose) polymerase family, member 10
Aliases:   FLJ14464
RefSeq:   NC_000008.10 NG_012492.1 NT_008046.16 NW_003315923.1
Ensembl:   ENSG00000178685
Entrez:   84875
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.710000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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