PrimePCR™ Probe Assay: CEP44, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053439
Assay Design:   exonic
Chromosome Location:   4:175220261-175223204question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000503780 ENST00000505124 ENST00000457424 ENST00000514712 ENST00000515299 ENST00000503053 ENST00000426172 ENST00000296519

Gene Information

Description Not Available

Gene Symbol:   CEP44
Gene Name:   centrosomal protein 44kDa
Aliases:   KIAA1712, PS1TP3
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164118
Entrez:   80817
UniGene:   Hs.555989
Chromosome Mapping:   4q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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