PrimePCR™ Probe Assay: PLCD3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLCD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLCD3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053208
Assay Design:   exonic
Chromosome Location:   17:43198601-43209631question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000564093 ENST00000375248 ENST00000579208 ENST00000314256 ENST00000372165 ENST00000372163 ENST00000334370 ENST00000251268 ENST00000593647 ENST00000378073 ENST00000263381 ENST00000599910 ENST00000389282 ENST00000545156 ENST00000306729 ENST00000361475 ENST00000536701 ENST00000563425 ENST00000395842 ENST00000308508 ENST00000587303 ENST00000372412 ENST00000357368 ENST00000262963 ENST00000348075 ENST00000353284 ENST00000380817 ENST00000348911 ENST00000390679 ENST00000307741 ENST00000585338 ENST

Gene Information

This gene encodes a member of the phospholipase C family which catalyze the hydrolysis of phosphatidylinositol 45-bisphosphate to generate the second messengers diacylglycerol and inositol 145-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine sphingosine and several phospholipids. [provided by RefSeq Jul 2008]

Gene Symbol:   PLCD3
Gene Name:   phospholipase C, delta 3
Aliases:   MGC71172
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000161714
Entrez:   113026
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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