PrimePCR™ Probe Assay: GTF3C6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GTF3C6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GTF3C6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052927
Assay Design:   exonic
Chromosome Location:   6:111288892-111289027question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000329970

Gene Information

RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g. GTF3C1 MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs including 5S RNA (MIM 180420) tRNA and adenovirus-associated (VA) RNA of both cellular and viral origin.[supplied by OMIM Mar 2008]

Gene Symbol:   GTF3C6
Gene Name:   general transcription factor IIIC, polypeptide 6, alpha 35kDa
Aliases:   C6orf51, TFIIIC35, bA397G5.3
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000155115
Entrez:   112495
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.150000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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