PrimePCR™ Probe Assay: ADAMTS8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051843
Assay Design:   exonic
Chromosome Location:   11:130275058-130275195question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000257359

Gene Information

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules including a propeptide region a metalloproteinase domain a disintegrin-like domain and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene most notably lung neoplasms. [provided by RefSeq Jul 2008]

Gene Symbol:   ADAMTS8
Gene Name:   ADAM metallopeptidase with thrombospondin type 1 motif, 8
Aliases:   ADAM-TS8, FLJ41712, METH2
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000134917
Entrez:   11095
Chromosome Mapping:   11q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.930000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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