PrimePCR™ Probe Assay: FHOD3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FHOD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FHOD3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051833
Assay Design:   exonic
Chromosome Location:   18:34335088-34335212question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000590592 ENST00000359247 ENST00000586635 ENST00000591635 ENST00000592930 ENST00000592128 ENST00000585579 ENST00000257209 ENST00000445677

Gene Information

Proteins that contain formin (FMN1; MIM 136535) homology (FH) domains such as FHOD3 play a role in regulation of the actin cytoskeleton (Kanaya et al. 2005 [PubMed 15966898]).[supplied by OMIM Apr 2010]

Gene Symbol:   FHOD3
Gene Name:   formin homology 2 domain containing 3
Aliases:   FHOS2, Formactin2
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000134775
Entrez:   80206
Chromosome Mapping:   18q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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