PrimePCR™ Probe Assay: ILKAP, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051692
Assay Design:   exonic
Chromosome Location:   2:239090738-239092317question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000254654 ENST00000450411

Gene Information

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1) a regulator of integrin mediated signaling and regulate the kinase activity of ILK. Through the interaction with ILK this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta) and thus participate in Wnt signaling pathway. [provided by RefSeq Jul 2008]

Gene Symbol:   ILKAP
Gene Name:   integrin-linked kinase-associated serine/threonine phosphatase
Aliases:   DKFZp434J2031, FLJ10181, MGC4846, PP2C-DELTA
RefSeq:   NC_000002.11 NT_005120.16
Ensembl:   ENSG00000132323
Entrez:   80895
UniGene:   Hs.92033
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.090000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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