PrimePCR™ Probe Assay: ELL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051497
Assay Design:   exonic
Chromosome Location:   15:44069308-44069438question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000319359 ENST00000433927 ENST00000381359

Gene Information

Description Not Available

Gene Symbol:   ELL3
Gene Name:   elongation factor RNA polymerase II-like 3
Aliases:   FLJ22637
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000128886
Entrez:   80237
UniGene:   Hs.706346
Chromosome Mapping:   15q15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.400000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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