PrimePCR™ Probe Assay: NXF5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NXF5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NXF5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051430
Assay Design:   exonic
Chromosome Location:   X:101097770-101098101question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000473265 ENST00000537026 ENST00000361708

Gene Information

This gene is one member of a family of nuclear RNA export factor genes. The encoded protein can bind RNA and is implicated in mRNA nuclear export. However this protein has lost several C-terminal protein domains found in other family members that are required for export activity and may be an evolving pseudogene. Alternatively spliced transcript variants have been described but most are candidates for nonsense-mediated decay (NMD) and may not express proteins in vivo. [provided by RefSeq Jul 2009]

Gene Symbol:   NXF5
Gene Name:   nuclear RNA export factor 5
Aliases:   Not Available
RefSeq:   NC_000023.10 NG_009851.1 NT_011651.17
Ensembl:   ENSG00000126952
Entrez:   55998
Chromosome Mapping:   Xq22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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