PrimePCR™ Probe Assay: CHD6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHD6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHD6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051278
Assay Design:   exonic
Chromosome Location:   20:40162051-40162175question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000373233 ENST00000373222 ENST00000440647 ENST00000309279

Gene Information

Chromosomal DNA of eukaryotic cells is compacted by nuclear proteins to form chromatin an organized nucleoprotein structure that can inhibit gene expression. Several multisubunit protein complexes exist to remodel the chromatin to allow patterns of cell type-specific gene expression. The protein encoded by this gene is thought to be a core member of one or more of these complexes. The encoded protein which is a member of the SNF2/RAD54 helicase family contains two chromodomains a helicase domain and an ATPase domain. [provided by RefSeq Jul 2008]

Gene Symbol:   CHD6
Gene Name:   chromodomain helicase DNA binding protein 6
Aliases:   CHD5, KIAA1335, RIGB
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000124177
Entrez:   84181
Chromosome Mapping:   20q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.330000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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