PrimePCR™ Probe Assay: IFT46, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051026
Assay Design:   exonic
Chromosome Location:   11:118427631-118428472question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000264021 ENST00000264020 ENST00000530872 ENST00000531939 ENST00000534156 ENST00000534114 ENST00000528378 ENST00000533918 ENST00000597276 ENST00000598014 ENST00000595543 ENST00000600698 ENST00000601455 ENST00000598210 ENST00000598778 ENST00000595675

Gene Information

Description Not Available

Gene Symbol:   IFT46
Gene Name:   intraflagellar transport 46 homolog (Chlamydomonas)
Aliases:   C11orf2, C11orf60, FLJ21827
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000118096
Entrez:   56912
UniGene:   Hs.533738
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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