PrimePCR™ Probe Assay: OSBPL9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: OSBPL9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OSBPL9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051019
Assay Design:   exonic
Chromosome Location:   1:52237851-52238395question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000371714 ENST00000447887 ENST00000428468 ENST00000453295 ENST00000531828 ENST00000361556 ENST00000462759 ENST00000486942 ENST00000371710 ENST00000337809 ENST00000530544 ENST00000435686

Gene Information

This gene encodes a member of the oxysterol-binding protein (OSBP) family a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants most of which encode distinct isoforms have been identified. Related pseudogenes have been identified on chromosomes 3 11 and 12. [provided by RefSeq Jul 2010]

Gene Symbol:   OSBPL9
Gene Name:   oxysterol binding protein-like 9
Aliases:   FLJ12492, FLJ14629, FLJ14801, FLJ32055, FLJ34384, MGC15035, ORP-9, ORP9
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000117859
Entrez:   114883
Chromosome Mapping:   1p32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.150000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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