PrimePCR™ Probe Assay: ERRFI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ERRFI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ERRFI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050942
Assay Design:   exonic
Chromosome Location:   1:8073966-8074109question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000377482 ENST00000467067 ENST00000469499

Gene Information

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al. 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33 which is induced during cell stress and mediates cell signaling (Makkinje et al. 2000 [PubMed 10749885]; Fiorentino et al. 2000 [PubMed 11003669]).[supplied by OMIM Mar 2008]

Gene Symbol:   ERRFI1
Gene Name:   ERBB receptor feedback inhibitor 1
Aliases:   GENE-33, MIG-6, MIG6, RALT
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000116285
Entrez:   54206
Chromosome Mapping:   1p36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.610000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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