PrimePCR™ Probe Assay: RASA4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RASA4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RASA4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050360
Assay Design:   exonic
Chromosome Location:   7:102240807-102242833question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000311413 ENST00000361246 ENST00000549558 ENST00000550693 ENST00000549493 ENST00000546568 ENST00000546960 ENST00000332712 ENST00000369446 ENST00000444565 ENST00000377451 ENST00000369505 ENST00000369445 ENST00000344532 ENST00000430805 ENST00000397041 ENST00000397035 ENST00000359983 ENST00000352011 ENST00000397043 ENST00000397039 ENST00000309890 ENST00000465829 ENST00000481893 ENST00000306682 ENST00000541662 ENST00000368426 ENST00000292176 ENST00000417934 ENST00000535420 ENST00000262940 ENST

Gene Information

This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane where it activates Ras GTPase activity. Consequently Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression cell growth and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RASA4
Gene Name:   RAS p21 protein activator 4
Aliases:   CAPRI, FLJ59070, GAPL, KIAA0538, MGC131890
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000105808
Entrez:   10156
Chromosome Mapping:   7q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.590000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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