PrimePCR™ Probe Assay: REC8, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050092
Assay Design:   exonic
Chromosome Location:   14:24641988-24642228question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000311457 ENST00000557806 ENST00000559919 ENST00000560501

Gene Information

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse the homologous protein is a key component of the meiotic cohesion complex which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   REC8
Gene Name:   REC8 homolog (yeast)
Aliases:   HR21spB, MGC950, REC8L1, Rec8p
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100918
Entrez:   9985
UniGene:   Hs.419259
Chromosome Mapping:   14q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.870000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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