PrimePCR™ Probe Assay: HOOK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HOOK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HOOK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049939
Assay Design:   exonic
Chromosome Location:   19:12883006-12883456question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000397668 ENST00000264827 ENST00000589400 ENST00000592079 ENST00000590839 ENST00000588052 ENST00000587964 ENST00000587178

Gene Information

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains which attach to microtubules and more divergent C-terminal domains which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM Apr 2004]

Gene Symbol:   HOOK2
Gene Name:   hook homolog 2 (Drosophila)
Aliases:   FLJ26218, HK2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000095066
Entrez:   29911
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.200000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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