PrimePCR™ Probe Assay: LIMA1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049390
Assay Design:   exonic
Chromosome Location:   12:50570948-50571073question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000552491 ENST00000547825 ENST00000552823 ENST00000394943 ENST00000341247 ENST00000552783 ENST00000552909

Gene Information

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene and expression of some of the variants maybe independently regulated. [provided by RefSeq Aug 2011]

Gene Symbol:   LIMA1
Gene Name:   LIM domain and actin binding 1
Aliases:   EPLIN, FLJ38853, MGC131726, SREBP3
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000050405
Entrez:   51474
UniGene:   Hs.525419
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 35.110000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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