PrimePCR™ Probe Assay: MPND, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MPND, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MPND, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049211
Assay Design:   exonic
Chromosome Location:   19:4359943-4360038question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000359600 ENST00000409379 ENST00000569563 ENST00000527009 ENST00000369888 ENST00000450537 ENST00000217130 ENST00000260447 ENST00000320891 ENST00000249373 ENST00000361373 ENST00000429277 ENST00000458213 ENST00000352360 ENST00000263066 ENST00000299441 ENST00000362042 ENST00000585291 ENST00000357480 ENST00000361665 ENST00000262418 ENST00000300590 ENST00000262966 ENST00000359935 ENST00000599840 ENST00000596722 ENST00000597036 ENST00000594162 ENST00000405592 ENST00000406652 ENST00000368385 ENST

Gene Information

Description Not Available

Gene Symbol:   MPND
Gene Name:   MPN domain containing
Aliases:   FLJ14981
RefSeq:   NC_000019.9 NG_028084.1 NT_011255.14
Ensembl:   ENSG00000008382
Entrez:   84954
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.690000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download