PrimePCR™ Probe Assay: JMJD7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: JMJD7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: JMJD7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041553
Assay Design:   exonic
Chromosome Location:   15:42129472-42129605question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000356842 ENST00000335712 ENST00000358510 ENST00000538214 ENST00000537768 ENST00000375279 ENST00000359606 ENST00000342704 ENST00000346541 ENST00000397800 ENST00000370602 ENST00000424789 ENST00000314636 ENST00000312233 ENST00000361115 ENST00000535144 ENST00000392973 ENST00000252032 ENST00000341313 ENST00000284425 ENST00000468660 ENST00000399908 ENST00000542534 ENST00000397299 ENST00000408047 ENST00000449956 ENST00000418710 ENST00000361443 ENST00000378561 ENST00000368773 ENST00000340740 ENST

Gene Information

Description Not Available

Gene Symbol:   JMJD7
Gene Name:   jumonji domain containing 7
Aliases:   FLJ20543, FLJ20656, FLJ20807, FLJ77014, FLJ78330
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000243789
Entrez:   100137047
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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