PrimePCR™ Probe Assay: TMEM110, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TMEM110, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TMEM110, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041088
Assay Design:   exonic
Chromosome Location:   3:52883808-52883905question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000342239 ENST00000324109 ENST00000413454 ENST00000596217 ENST00000597723 ENST00000597029 ENST00000352066 ENST00000422090 ENST00000468789 ENST00000436083 ENST00000356987 ENST00000432564 ENST00000263207 ENST00000401994 ENST00000406522 ENST00000406259 ENST00000344269 ENST00000371897 ENST00000371899 ENST00000276816 ENST00000394909 ENST00000252723 ENST00000566936 ENST00000340099 ENST00000046640 ENST00000381870 ENST00000441220 ENST00000414524 ENST00000462833 ENST00000491190 ENST00000309879 ENST

Gene Information

Description Not Available

Gene Symbol:   TMEM110
Gene Name:   transmembrane protein 110
Aliases:   DKFZp667E1121, DKFZp779M0254, FLJ37613, FLJ42395, MGC52022
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000213533
Entrez:   375346
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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