PrimePCR™ Probe Assay: SSX4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SSX4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SSX4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041061
Assay Design:   exonic
Chromosome Location:   X:48244794-48244887question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000298181 ENST00000336777 ENST00000337502 ENST00000375515 ENST00000276049 ENST00000280481 ENST00000371561 ENST00000315048 ENST00000376923 ENST00000311798 ENST00000347757 ENST00000376919 ENST00000298396 ENST00000376893 ENST00000376886 ENST00000375517 ENST00000376884 ENST00000396928 ENST00000361445 ENST00000405592 ENST00000406652 ENST00000594778 ENST00000594016 ENST00000596327 ENST00000596574 ENST00000596036 ENST00000594782 ENST00000595689 ENST00000595235 ENST00000598733 ENST00000593457 ENST

Gene Information

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1 SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma X breakpoint 4 SSX4 and SSX4B in tail-to-tail orientation. This gene SSX4 represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SSX4
Gene Name:   synovial sarcoma, X breakpoint 4
Aliases:   CT5.4, MGC119056, MGC12411, SSX4B
RefSeq:   NC_000023.10 NG_005575.1 NT_079573.4
Ensembl:   ENSG00000204645
Entrez:   6759
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 36.790000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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