PrimePCR™ Probe Assay: JMJD7-PLA2G4B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: JMJD7-PLA2G4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: JMJD7-PLA2G4B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040869
Assay Design:   exonic
Chromosome Location:   15:42139886-42139975question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000393359 ENST00000258449 ENST00000325285 ENST00000397641 ENST00000215862 ENST00000261383 ENST00000415178 ENST00000370571 ENST00000436319 ENST00000453269 ENST00000472509 ENST00000452041 ENST00000344095 ENST00000437822 ENST00000418432 ENST00000371589 ENST00000535144 ENST00000474902 ENST00000294638 ENST00000356261 ENST00000559413 ENST00000525144 ENST00000529097 ENST00000525704 ENST00000382448 ENST00000342159 ENST00000458483 ENST00000452633 ENST00000300051 ENST00000450168 ENST00000507166 ENST

Gene Information

This gene transcribes naturally-occurring mRNAs that are read-through products of the neighboring jumonji domain containing 7 (JMJD7) and phospholipase A2 group IVB (PLA2G4B) genes. These read-through products contain in-frame segments from both genes but a function has yet to be determined for the predicted fusion proteins which contain a partial JmjC domain and downstream C2 and phospholipase A2 domains. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   JMJD7-PLA2G4B
Gene Name:   JMJD7-PLA2G4B readthrough
Aliases:   HsT16992, cPLA2-beta
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000168970
Entrez:   8681
Chromosome Mapping:   15q11.2-q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.100000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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