This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: JMJD7-PLA2G4B, Human
PrimePCR™ Template for Probe Assay: JMJD7-PLA2G4B, Human
This gene transcribes naturally-occurring mRNAs that are read-through products of the neighboring jumonji domain containing 7 (JMJD7) and phospholipase A2 group IVB (PLA2G4B) genes. These read-through products contain in-frame segments from both genes but a function has yet to be determined for the predicted fusion proteins which contain a partial JmjC domain and downstream C2 and phospholipase A2 domains. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.