PrimePCR™ Probe Assay: SF1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040863
Assay Design:   exonic
Chromosome Location:   11:64535067-64535194question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000339591 ENST00000449880 ENST00000450894 ENST00000200181 ENST00000579662 ENST00000396219 ENST00000352665 ENST00000299764 ENST00000374846 ENST00000352456 ENST00000595018 ENST00000392006 ENST00000378215 ENST00000602130 ENST00000593587 ENST00000263367 ENST00000599614 ENST00000263275 ENST00000393313 ENST00000265827 ENST00000377387 ENST00000377390 ENST00000227503 ENST00000377394 ENST00000334944 ENST00000443908 ENST00000433274 ENST00000486867 ENST00000422298 ENST00000502732 ENST00000512653 ENST

Gene Information

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq May 2010]

Gene Symbol:   SF1
Gene Name:   splicing factor 1
Aliases:   BBP, D11S636, MBBP, ZFM1, ZNF162
RefSeq:   NC_000011.9 NG_013018.1 NT_167190.1 NG_021399.1
Ensembl:   ENSG00000168066
Entrez:   7536
UniGene:   Hs.502829
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download