PrimePCR™ Probe Assay: DCLK1, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040762
Assay Design:   exonic
Chromosome Location:   13:36428636-36445371question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000379893 ENST00000255448 ENST00000360631 ENST00000379892 ENST00000358070 ENST00000338081 ENST00000356220 ENST00000488120 ENST00000356915 ENST00000371993 ENST00000395530

Gene Information

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains which bind microtubules and regulate microtubule polymerization a C-terminal serine/threonine protein kinase domain which shows substantial homology to Ca2+/calmodulin-dependent protein kinase and a serine/proline-rich domain in between the doublecortin and the protein kinase domains which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes including neuronal migration retrograde transport neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms which are differentially expressed and have different kinase activities.[provided by RefSeq Sep 2010]

Gene Symbol:   DCLK1
Gene Name:   doublecortin-like kinase 1
Aliases:   CL1, CLICK1, DCAMKL1, DCDC3A, DCLK, KIAA0369
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000133083
Entrez:   9201
UniGene:   Hs.507755
Chromosome Mapping:   13q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.