PrimePCR™ Probe Assay: EPS8L1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EPS8L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EPS8L1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040753
Assay Design:   exonic
Chromosome Location:   19:55598896-55598998question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000381884 ENST00000545981 ENST00000586329 ENST00000201647 ENST00000540810 ENST00000588359 ENST00000245618 ENST00000590429 ENST00000572133 ENST00000574843 ENST00000586333 ENST00000572780 ENST00000588252 ENST00000570966 ENST00000571582 ENST00000585743 ENST00000573442 ENST00000589832 ENST00000576261 ENST00000575138 ENST00000592799 ENST00000571474 ENST00000592856 ENST00000577025 ENST00000575720 ENST00000590182 ENST00000576005 ENST00000587643 ENST00000575092 ENST00000576308 ENST00000585787 ENST

Gene Information

This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8) a substrate for the epidermal growth factor receptor. The function of this protein is unknown. At least two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   EPS8L1
Gene Name:   EPS8-like 1
Aliases:   DRC3, EPS8R1, FLJ20258, MGC23164, MGC4642
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000131037
Entrez:   54869
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.270000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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