PrimePCR™ Probe Assay: RP11-1286E23.7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040158
Assay Design:   exonic
Chromosome Location:   4:9222889-9223005question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000515566 ENST00000506151 ENST00000506414 ENST00000511280 ENST00000504481 ENST00000509271 ENST00000509660 ENST00000507227 ENST00000515574 ENST00000504543 ENST00000511681 ENST00000504104 ENST00000333796 ENST00000429667 ENST00000421288 ENST00000417945 ENST00000457736 ENST00000415041 ENST00000456464 ENST00000511568 ENST00000504209 ENST00000409681 ENST00000263857

Gene Information

Description Not Available

Gene Symbol:   RP11-1286E23.7
Gene Name:   Ubiquitin carboxyl-terminal hydrolase
Aliases:   Not Available
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000227551
Entrez:   100287205
UniGene:   Not Available
Chromosome Mapping:   4p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.600000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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