PrimePCR™ Probe Assay: P2RY6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: P2RY6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: P2RY6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039519
Assay Design:   exonic
Chromosome Location:   11:73008075-73008185question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000540342 ENST00000542092 ENST00000349767 ENST00000393592 ENST00000393591 ENST00000540124 ENST00000393590 ENST00000536225 ENST00000535931 ENST00000538328

Gene Information

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity which overlaps in some cases for various adenosine and uridine nucleotides. This receptor is responsive to UDP partially responsive to UTP and ADP and not responsive to ATP. Four transcript variants encoding the same isoform have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   P2RY6
Gene Name:   pyrimidinergic receptor P2Y, G-protein coupled, 6
Aliases:   MGC15335, P2Y6
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000171631
Entrez:   5031
Chromosome Mapping:   11q13.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.510000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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