PrimePCR™ Probe Assay: STEAP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039401
Assay Design:   exonic
Chromosome Location:   7:89854499-89854640question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000428074 ENST00000287908 ENST00000394626 ENST00000394622 ENST00000394632 ENST00000426158 ENST00000402625 ENST00000394629 ENST00000394621

Gene Information

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex the plasma membrane and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   STEAP2
Gene Name:   six transmembrane epithelial antigen of the prostate 2
Aliases:   IPCA1, PCANAP1, PUMPCn, STAMP1, STMP
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000157214
Entrez:   261729
UniGene:   Hs.489051
Chromosome Mapping:   7q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.370000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download