This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CXorf1, Human
PrimePCR™ Template for Probe Assay: CXorf1, Human
This intronless gene is expressed in the hippocampus and maps close to a candidate region for several X-linked mental retardation (XLMR) syndromes. It is conserved in primates cow and horse but not found in mouse and rat. The exact function of this gene is not known but on the basis of its physical location and expression pattern it is proposed to have an important function in the brain. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.