PrimePCR™ Probe Assay: KBTBD13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KBTBD13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KBTBD13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032018
Assay Design:   Exonic
Chromosome Location:   15:65370902-65371048question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000432196

Gene Information

The gene belongs to a family of genes encoding proteins containing a BTB domain and several kelch repeats. The BTB domain functions as a protein-protein interaction module which includes an ability to self-associate or to interact with non-BTB domain-containing proteins. The kelch motif typically occurs in groups of five to seven repeats and has been found in proteins with diverse functions. Known functions of these family members include transcription regulation ion channel tetramerization and gating protein ubiquitination or degradation and cytoskeleton regulation. The exact function of this family member has yet to be determined. [provided by RefSeq Jun 2010]

Gene Symbol:   KBTBD13
Gene Name:   kelch repeat and BTB (POZ) domain containing 13
Aliases:   HCG1645727, NEM6
RefSeq:   NC_000015.9 NG_021411.1 NT_010194.17
Ensembl:   ENSG00000234438
Entrez:   390594
Chromosome Mapping:   15q22.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.250000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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