PrimePCR™ Probe Assay: RIPK3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RIPK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RIPK3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025866
Assay Design:   Exonic
Chromosome Location:   14:24805514-24806107question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000216274

Gene Information

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq Jul 2008]

Gene Symbol:   RIPK3
Gene Name:   receptor-interacting serine-threonine kinase 3
Aliases:   RIP3
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000129465
Entrez:   11035
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.330000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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