PrimePCR™ Probe Assay: IKZF3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IKZF3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IKZF3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025808
Assay Design:   Exonic
Chromosome Location:   17:37922072-37922204question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000488188 ENST00000439167 ENST00000377945 ENST00000394189 ENST00000377944 ENST00000377958 ENST00000439016 ENST00000377952 ENST00000351680 ENST00000346243 ENST00000350532 ENST00000467757 ENST00000346872 ENST00000535189

Gene Information

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers Ikaros/Aiolos heterodimers and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described as well as some non-protein coding variants. [provided by RefSeq Apr 2012]

Gene Symbol:   IKZF3
Gene Name:   IKAROS family zinc finger 3 (Aiolos)
Aliases:   AIO, AIOLOS, ZNFN1A3
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000161405
Entrez:   22806
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.720000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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