PrimePCR™ Probe Assay: ANKRD6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025717
Assay Design:   Exonic
Chromosome Location:   6:90276719-90276810question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000369408 ENST00000339746 ENST00000447838 ENST00000520458 ENST00000465722 ENST00000523798 ENST00000522441 ENST00000522779 ENST00000485637 ENST00000522705 ENST00000518150 ENST00000520793

Gene Information

Description Not Available

Gene Symbol:   ANKRD6
Gene Name:   ankyrin repeat domain 6
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000135299
Entrez:   22881
UniGene:   Hs.702213
Chromosome Mapping:   6q14.2-q16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.800000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download