PrimePCR™ Probe Assay: HOXC6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024822
Assay Design:   Exonic
Chromosome Location:   12:54423502-54423674question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000394331 ENST00000243108

Gene Information

This gene belongs to the homeobox family members of which encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters HOXA HOXB HOXC and HOXD which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene HOXC6 is one of several HOXC genes located in a cluster on chromosome 12. Three genes HOXC5 HOXC4 and HOXC6 share a 5' non-coding exon. Transcripts may include the shared exon spliced to the gene-specific exons or they may include only the gene-specific exons. Alternatively spliced transcript variants encoding different isoforms have been identified for HOXC6. Transcript variant two includes the shared exon and transcript variant one includes only gene-specific exons. [provided by RefSeq Jul 2008]

Gene Symbol:   HOXC6
Gene Name:   homeobox C6
Aliases:   CP25, HHO.C8, HOX3, HOX3C
RefSeq:   NC_000012.11 NG_005542.1 NT_029419.12
Ensembl:   ENSG00000197757
Entrez:   3223
UniGene:   Hs.549040
Chromosome Mapping:   12q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download