PrimePCR™ Probe Assay: OS9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024769
Assay Design:   Exonic
Chromosome Location:   12:58112012-58112114question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000552285 ENST00000315970 ENST00000439210 ENST00000389146 ENST00000413095 ENST00000551035 ENST00000257966 ENST00000435406 ENST00000389142

Gene Information

This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1) a key regulator of the hypoxic response and angiogenesis and promotes the degradation of one of its subunits. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   OS9
Gene Name:   osteosarcoma amplified 9, endoplasmic reticulum lectin
Aliases:   ERLEC2, OS-9
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135506
Entrez:   10956
UniGene:   Hs.527861
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.250000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download