PrimePCR™ Probe Assay: GPR55, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024136
Assay Design:   Exonic
Chromosome Location:   2:231775345-231775513question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000392039 ENST00000438398 ENST00000392040

Gene Information

Members of the G protein-coupled receptor (GPR) family such as GPR55 play important roles in signal transduction from the external environment to the inside of the cell (Sawzdargo et al. 1999 [PubMed 9931487]).[supplied by OMIM Apr 2008]

Gene Symbol:   GPR55
Gene Name:   G protein-coupled receptor 55
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000135898
Entrez:   9290
UniGene:   Hs.114545
Chromosome Mapping:   2q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.100000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download