PrimePCR™ SYBR® Green Assay: POLR2J3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0020588
Assay Design:   Exonic
Chromosome Location:   7:102207065-102207157question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000513506 ENST00000514917 ENST00000511313

Gene Information

This gene is a member of the RNA polymerase II subunit 11 gene family which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family DNA directed RNA polymerase II polypeptide J has been shown to encode a subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq Jul 2008]

Gene Symbol:   POLR2J3
Gene Name:   polymerase (RNA) II (DNA directed) polypeptide J3
Aliases:   MGC54043, POLR2J2, RPB11b1, RPB11b2
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000168255
Entrez:   548644
UniGene:   Hs.656696
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.500000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download