Gene:  POLR2J3, Human

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This gene is a member of the RNA polymerase II subunit 11 gene family which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family DNA directed RNA polymerase II polypeptide J has been shown to encode a subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0020588
Info:   Recommended - best specificity
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056874
Info:   Same primer pair as used in probe assay qHsaCEP0057979
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057979
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5004792
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0020588; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189468
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0056874; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192627
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0057979; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POLR2J3, Human
polymerase (RNA) II (DNA directed) polypeptide J3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192628
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0057979; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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