PrimePCR™ SYBR® Green Assay: RAP1GAP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0024849

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0003852
Assay Design:   Exonic
Chromosome Location:   1:21940121-21940503question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000374765 ENST00000374763 ENST00000359708 ENST00000374757 ENST00000290101 ENST00000374761 ENST00000542643 ENST00000374758

Gene Information

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene RAP1GAP promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins guanine nucleotide exchange factors (GEFs) act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general ras subfamily proteins such as RAP1 play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation adhesion differentiation and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq Aug 2011]

Gene Symbol:   RAP1GAP
Gene Name:   RAP1 GTPase activating protein
Aliases:   KIAA0474, RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000076864
Entrez:   5909
UniGene:   Hs.148178
Chromosome Mapping:   1p36.1-p35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 38.010000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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