PrimePCR™ Probe Assay: SHD, Arabidopsis

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SHD, Arabidopsis
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SHD, Arabidopsis
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qAthCEP0026494
Assay Design:   Exonic
Chromosome Location:   4:12553005-12553119question
Amplicon Length:   85
Splice Variants Targeted:   AT5G51130.1 AT4G24190.1 AT4G24190.2

Gene Information

encodes an ortholog of GRP94, an ER-resident HSP90-like protein and is involved in regulation of meristem size and organization. Single and double mutant analyses suggest that SHD may be required for the correct folding and/or complex formation of CLV proteins. Lines carrying recessive mutations in this locus exhibits expanded shoot meristems, disorganized root meristems, and defective pollen tube elongation. Transcript is detected in all tissues examined and is not induced by heat. Endoplasmin supports the protein secretory pathway and has a role in proliferating tissues.

Gene Symbol:   SHD
Gene Name:   Chaperone protein htpG family protein
Aliases:   Not Available
RefSeq:   NM_118552 NM_202877
Ensembl:   AT4G24190
Entrez:   828520
Chromosome Mapping:   Not Available

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